PLoS ONE 8(10):Įditor: Martin Heil, Centro de Investigación y de Estudios Avanzados, Mexico This is the first work analysing the sequence of multiple loci to reveal geno- and ecotypes of nectar bacteria.Ĭitation: Álvarez-Pérez S, de Vega C, Herrera CM (2013) Multilocus Sequence Analysis of Nectar Pseudomonads Reveals High Genetic Diversity and Contrasting Recombination Patterns.
Analyses relating diversification to floristic region and pollinator type revealed that there has been more unique evolution of the nectar pseudomonads within the Mediterranean region than would be expected by chance.
In contrast, the other two NGs comprised both South African and Mediterranean isolates. Nevertheless, signatures of recombination were observed for NG 3, which exclusively included isolates retrieved from the floral nectar of insect-pollinated Mediterranean plants. Linkage disequilibrium analysis pointed to a mostly clonal population structure, even when the analysis was restricted to isolates from the same floristic region or belonging to the same NG. Phylogenetic analyses resulted in the identification of three main clades of nectar pseudomonads, which were closely related to five intrageneric groups: Pseudomonas oryzihabitans (NG 1) P. A total of 35 different sequence types were found for the 38 nectar bacterial isolates characterised. The genetic and evolutionary relationships among floral nectar-dwelling Pseudomonas ‘sensu stricto’ isolates associated to South African and Mediterranean plants were investigated by multilocus sequence analysis (MLSA) of four core housekeeping genes ( rrs, gyrB, rpoB and rpoD).
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